Ecophysiology of Nitrogen Fixation Ability on 3 cultivars Common Bean (Phaseolus vulgaris L.) with some types of Inoculants which contain different strains of Rhizobium leguminosarum; bv.phaseoli

Document Type : Original Article

Author

Islamic Azad University of Miyaneh Branch. Iran

Abstract

Nitrogen
Fixation Ability on Common Bean (Phaseolus vulgaris L.) cultivars with
Some types of Inoculants which contain different strains of Rhizobium leguminosarum; bv. phaseoliwas
evaluated in an
experimental design. In this investigation three types of biological
fertilizers such as Rb117, Rb123, Rb136 and a commercial inoculants (Rhizobean
super plus) with two treatment N100 (use of 100 Kg nitrogen/ha) and control (without
seeds inoculated and fertilizer) and three bean cultivars: COS16 (spotted
bean), Derakhshan and Akhtar (red beans) used as factorial experiment in
randomized complete block design in 2006 and 2007 in Zanjan
province(Khoramdareh Region). Combined Analysis preformed with SAS9.2 of
statistical program and graphs were provided by Excel and Σplot
programs and means comparison were done with Duncan,s Multiple Range Test. Rb117 strain was showed an
59 percent increasing in seed yield compared with non-inoculants treatment.
Among of inoculants the effect of Rb123 strain, was at least (32%) in compared
with others. The results showed that significant difference in seed yield,
nitrogen percent per plant, seed protein percentage, number and weight of
nodules (50 day after emergence),seed and dry matter yield were observed among
seed inoculated and non-inoculated. Most protein percent were achieved from 100
kg nitrogen, and Rb117 treatment (About 24%) and highest protein yield (864
kg/ha) was gained from Rb117 and lowest protein yield was produced from
control. Among of all inoculants (or strains of Rhizobium leguminosarum;bv.phaseoli) The  Rb117 
strain has more effectiveness on common bean, as compared with other
strains. In three cultivars the COS16 line was successful in
compared with other cultivars. High seed yield and many evaluated traits
achieved from COS16 line (spotted bean), second year of experiment and Rb117
strain. This investigation showed that to achievement for satisfying yield and
sustainable agriculture, all of industrial bio fertilizers must be used as
complementary materials with mineral fertilizers

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