Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences, H. Botany
2090-3812
2090-3820
10
1
2019
06
01
Inter-Specific Variation in SDS-PAGE Electrophograms of Total Leaf Proteins in Some Species of Subtribe Cassiinae
1
11
EN
Kolawole
O.
S.
1-Department of Biological Sciences, Faculty of Science, Federal University, Kashere, Gombe State.
2-Applied Plant Anatomy and Wood Technology Laboratory,
Department of Plant Biology, Faculty of Life Sciences, University of Ilorin, Ilorin
kolawoleopeyemisaheed@gmail.com
Abdulrahaman
A.
A.
Applied Plant Anatomy and Wood Technology Laboratory,
Department of Plant Biology, Faculty of Life Sciences, University of Ilorin, Ilorin.
10.21608/eajbsh.2019.29965
The study employs the SDS-PAGE electrophoretic techniques to evaluate the taxonomic implications of some species in the subtribe Cassiinae(Caesalpinaceae) using total leaf proteins. The aim was to assess the genetic variation and relationship among the 15 species of subtribe Cassiinaethrough electrophoretic studies of their leaf proteins. Total leaf proteins were extracted and separated on 12% polyacrylamide gels using standard protocols. Young leaves (0.8g) of the plants were washed with distilled water and macerated with sterile mortar and pestle in 0.8% Phosphate Buffer-Saline (PBS) containing 0.4M NaCl at PH 8.0. Results obtained revealed that protein pattern was taxon-specific as no two species have the same banding pattern. Distant polymorphism in electrophoretic banding patterns of the leaf was observed through a total of forty-one (41) polypeptide bands. Variation existed not only in the number of bands but also in the intensity of bands in the leaf samples studied. The coefficients of similarity range between 0.076 – 0.845. The hierarchical cluster analysis (dendrogram) for the 15 species revealed two major clusters. The first group are <em>Senna</em> <em>spectabilis</em>, <em>S. alata</em>, <em>S. hirsuta</em>, <em>Chamaecrista rotundifolia</em>, <em>C. mimosoides</em>, <em>S. biflora</em> and the second group are <em>S. podocarpa</em>, <em>S. sophera</em>, <em>S. occidentalis</em>, <em>S. obtusifolia</em>, <em>Cassia italica</em>, <em>S. siamea</em>, <em>C. singueana,</em> <em>C. sieberiana</em> and <em>C. fistula</em>. An artificial key for the studied species of subtribe Cassiinae based on their band relationships is also provided. Therefore, the number and intensity of bands are additional characters that can be used for species delimitation in subtribe Cassiinae.
Cassiinae,Clustering,SDS-PAGE,Leaf protein,delimitation
https://eajbsh.journals.ekb.eg/article_29965.html
https://eajbsh.journals.ekb.eg/article_29965_40b59463bd94fcaee29f89f0a039ab74.pdf
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences, H. Botany
2090-3812
2090-3820
10
1
2019
06
01
Morphological, Biochemical and Soluble Sugars Characters of Aloe vera Subjected to Saline Condition
13
23
EN
Negahdari
Sedigheh
Department of Plant Biology, Faculty of Life Sciences, University of Ilorin, Ilorin.
deylaman_985@yahoo.com
10.21608/eajbsh.2019.29966
<strong>Aims</strong>:Salinity is one of the factors limiting the growth and survival of the plants, stops their growth by interactions such as osmotic potential and toxicity and makes the nutrition imbalance.<br /> <strong>Methods</strong>: The research examined the salinity effect on pharmaceutical <em>Aloe Barbadensis</em> plant; the plant was irrigated with half percent Hoagland nutritional solution containing different rates of Sodium chloride (0, 6, 12 & 18 dS/m) and the plants were harvested in 15 and 45 day intervals.<br /> <strong>Results</strong>:The following morphologic specifications were tested: the plant height, the leaf and root length, the leaf thickness and breadth, the weight of wet shoot and root, the weight of dry shoot and root and the weight of the gel and gel soluble sugars in Aloe vera leaves. In addition, the concentration of Na, K, Cl and Ca of the shoot and root and K/Na and Ca/ Na ratio were examined.<br /> <br /> <br /> <br /> <br /> <br /> <strong>Conclusions</strong>:One of the most important Aloe Vera's tolerance mechanisms may be the creation of appropriate K/Na , Ca/Na ratio in the shoot in primary time of the tension namely in 15 days and also the salinity rates create many fluctuations on the soluble sugars rates especially glucose and xylose
sodium,Chlorine,Nutritional solution,Salinity resistance,Aloe vera
https://eajbsh.journals.ekb.eg/article_29966.html
https://eajbsh.journals.ekb.eg/article_29966_4d3b74f66bf410d52dc7b2966e674462.pdf
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences, H. Botany
2090-3812
2090-3820
10
1
2019
06
01
Performance of Some Egyptian Rice Cultivars under Different Potassium Fertilization Rates
25
33
EN
Samah
M.
Aamer
Rice Research and Training Center (RRTC), Sakha, 33717, Kafrelsheikh, Egypt
Dalia
M.
Tabl
Rice Research Technology Center (RTTC), Field Crops Research Institute, Agricultural Research Center, Alexandria, Egypt
mohdalia4e@gmail.com
10.21608/eajbsh.2019.31757
Two field experiments were conducted at the Experimental Farm of the Rice Research and Training Center (RRTC), Sakha Research Station, Kafrelsheikh, Egypt, during two seasons of 2016 and 2017 to study the response of some rice cultivars to some potassium rates. The experimental design was a split- plot system with three replicates. The main plots were occupied by the eight rice cultivars “Sakha 101, Sakha102, Sakha105, Sakha106, Giza 177, Giza178, Giza179 and Giza 182”. While, the sub-plots contain four Potassium rates (0, 50, 100and 150% from recommended of K=RDK). Panicle length (cm), number of filled grains/panicle, 1000- grains weight (g), biological, grain, and straw yields (t/ha.), hulling %, milling %, GT%, amylose, grain K content, grain protein content (%) were determined in both seasons. The obtained results revealed that all cultivars were affected significantly regard their respect to K fertilization due to their genetically differed. Moreover, the rice cultivars Giza 178, Sakha 101 and 102 had a significant response with increasing K- fertilizer rate up to 150 from the recommended dose (RDK) as compared with other cultivars under the study
rice,varieties,yield,potassium,fertilization,rates
https://eajbsh.journals.ekb.eg/article_31757.html
https://eajbsh.journals.ekb.eg/article_31757_2f6f5f3f1484997298650435ba103599.pdf
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences, H. Botany
2090-3812
2090-3820
10
1
2019
06
01
Response of Wheat Plants to Seaweed Extracts and Fluvic Acid under Irrigation with Drainage Water
35
44
EN
Gomaa
M.
A.
Plant production Department, The Faculty of Agriculture (Saba Basha), Alexandria University
E.
E.
Kandi
Plant production Department, The Faculty of Agriculture (Saba Basha), Alexandria University
essam.kandil@gmail.com
A.
F.
Gharib
Plant production Department, The Faculty of Agriculture (Saba Basha), Alexandria University
10.21608/eajbsh.2019.44518
<span>Two field experiments were conducted at the Rosetta region, El_Behira Governorate, Egypt, during 2017/2018 and 2018/2019 growing seasons to study the effect of water quality, fulvic acid and seaweed extracts on the yield, its components and quality characters of wheat plant (Giza 168), in split-split plot design with three replications. The main plots included irrigation water quality (Nile water and Agricultural drainage water), while seaweed extracts (control, 50 and 100 g SW/fed) was arranged in the subplot. While fulvic acid application (control, 1, 2 kg FA/fed) occupied the sub-subplot. The results concluded that application of water quality, seaweed extracts and their interaction affected significantly wheat yield and its components, where yield and its components of wheat crop increased with using Nile water (high quality water), or drainage water with foliar application of seaweed extracts (SWE) at the rate of 100 g/fed and soil application of fulvic acid at the rate of 2 kg/fed</span>
Wheat,Irrigation,water,Quality,fulvic aid,seaweed extracts,yield,grain
https://eajbsh.journals.ekb.eg/article_44518.html
https://eajbsh.journals.ekb.eg/article_44518_8df13d38d83ddffc40e0ff16c7591584.pdf
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences, H. Botany
2090-3812
2090-3820
10
1
2019
06
01
Multivariate Analyses of Physicochemical Factors Controlling Cyanobacteria Biodiversity in Al-Lith Thermal Springs, KSA.
45
58
EN
Hatem
E. M.
Abdelwahab
1- Faculty of Sciences and Arts, khulais branch, University of Jeddah Jeddah, KSA.
2- Faculty of Science, Suez Canal University, Egypt
Abeer
S.
Amin
-Faculty of Sciences and Arts, khulais branch, University of Jeddah Jeddah, KSA.
-Faculty of Science, Suez Canal University, Egypt
abeeramin2003@yahoo.com
10.21608/eajbsh.2019.47276
<span>Nowadays thermal springs are worldwide known sources of valuable Biobricks for biotechnological applications. The ecological analysis is a prerequisite to exploring thermophiles cyanobacteria that are a pervasive group in thermal springs globally. Therefore, this study aims to ecologically analyze cyanobacteria in Al-Lith area to extract growth factors that control prokaryotic primary producers and species biodiversity. Previously, there are four known thermal spring in Al-Lith, Saudi Arabia. This study discovered additional six novel springs that were never mentioned before. A seasonal sampling of cyanobacteria and physicochemical factors carried out from winter, 2015 to spring of 2016 in order to analyze factors controlling cyanobacteria species diversity. Temperature, total dissolved salt (TDS), pH, and dissolved oxygen and 17 chemical elements were investigated. A total of 34 species were identified in Al-Lith springs. Ghomygah thermal spring is the largest spring in Al-Lith region that is rich in high diversity of cyanobacteria. Oscillatoria and Chroococcus are dominant species and functionally tuned to all thermal habitat in Al-Lith springs regardless of the different growth conditions among them. The multivariate analysis helps resolve the complex relationships between physicochemical parameters and cyanobacteria biodiversity. Temperature is the pre-eminent factor determine the distribution of mat-forming cyanobacteria in spite of the difference in other factors. The remarkable increase in pH was observed with increases in cyanobacteria coverage. Therefore, pH is directly controlled by cyanobacteria. Thermal springs in Al-Lith grouped primarily based on physicochemical characteristics and secondly based on their geographical location. This work provides a comprehensive characterization of thermophiles native habitat that formulate a foundation for biotechnological deployment of cyanobacteria. Indeed, rational conservation of operative and healthy ecosystems are imperative to preserve these valuable resources.</span>
Thermal springs,thermophiles – cyanobacteria – Al-Lith – Saudi Arabia – physicochemical factors
https://eajbsh.journals.ekb.eg/article_47276.html
https://eajbsh.journals.ekb.eg/article_47276_5672e017bfd82e862f35de49c470a95e.pdf
Egyptian Society of Biological Sciences
Egyptian Academic Journal of Biological Sciences, H. Botany
2090-3812
2090-3820
10
1
2019
06
01
Maize Hybrids Response to Nitrogen, Potassium Fertilization and Its Relation to Some Fungal Diseases
59
68
EN
Gomaa
M.
A.
Plant Production Department, Faculty of Agriculture (Saba Basha), Alexandria University, Egypt.
I.
F.
Rehab
Plant Production Department, Faculty of Agriculture (Saba Basha), Alexandria University, Egypt.
E.
E.
Kandi
Plant Production Department, Faculty of Agriculture (Saba Basha), Alexandria University, Egypt
essam.kandil@googlemail.com
A.
I. A.
Heflish
Agricultural Botany Department, Faculty of Agriculture (Saba Basha), Alexandria University, Egypt.
B.
.M.
Hamady
Plant Production Department, Faculty of Agriculture (Saba Basha), Alexandria University, Egypt
10.21608/eajbsh.2019.47641
Two field experiments were conducted at the Experimental Farm of Faculty of Agriculture (Saba Basha), Alexandria University, Egypt during 2017 and 2018 seasons, to study the response of the three maize hybrids productivity to different combination between nitrogen and potassium fertilizer and its effect to infection by fungal diseases. Split plot design with three replications was used, where the main plots occupied by three hybrids of maize (S.C.10, S.C.131 and T.W.C.321), meanwhile the subplots contained combination between nitrogen and potassium fertilizer forms (192 kg N/ha + 0 kg K/ha, 192 kg N + 57.6 kg K/ha, 192 kg N + 115.2 kg K/ha, 288 kg N/ha + 0 kg K/ha, 288 kg N + 57.6 kg K/ha, 288 kg N + 115.2 kg K/ha, 384 kg N/ha + 0 kg K/ha, 384 kg N + 57.6 kg K/ha, and 384 kg N + 115.2 kg K/ha). Each subplot consisted of 6 ridges 3.50 m in length and 70 cm in the width and plot area was 14.7 m<sup>2</sup>. The results revealed that maize hybrids differed significantly and combination between nitrogen and potassium fertilizer affected significantly in plant height (cm), ear length, number grains/row, number of grains/ears, 100- grain weight, grain, straw, and biological yield (kg/ha.) as well as harvest index %. The results indicated that there was significant effect of fertilization levels on yield, its components and leaf blight infestation of some maize hybrids where fertilizing maize hybrid TWC321 by 288 kg N + 57.6 kg K/ha recorded the highest values of yield and its components, on the other hand, the maize hybrid TWC321 recorded the highest yield and high tolerant for leaf blight under Alexandria conditions.
Maize,nitrogen,potassium,yield,composition,fungal diseases
https://eajbsh.journals.ekb.eg/article_47641.html
https://eajbsh.journals.ekb.eg/article_47641_8b3de0bbb57d61604278cdca5094cfd1.pdf