Determination of TaSC salt tolerance gene expression in selected wheat under different salt stresses

M., Majeed, D.; S., Shwkat M.; A., Sabbah M.

doi:10.21608/eajbsh.2014.16834

Determination of TaSC salt tolerance gene expression in selected wheat under different salt stresses

Document Type : Original Article

Authors

¹ Biotechnology research center, Alnahrain University, Baghdad, Iraq

² Biotechnology Department, College of Science/ Baghdad University

³ Forensic DNA Center, Alnahrain University, Baghdad, Iraq

10.21608/eajbsh.2014.16834

Abstract

In previous studies TaSC gene was reported to selectively express in salt tolerance wheat. This work was conducted to reveal the TaSC gene expression in Iraqi selected wheat genotypes (Triticum asetivium) (Dijilla, Furat and N3) and local cultivar (Tamoose) under different salinity levels (0, 10, 16 and 20) ds/m, using real-time polymers chain reaction. RNA was extracted from leaves and roots then converted to cDNA which amplified with pairs of primers and probe. The results indicated that the selected genotypes (Dijilla, Furat and N3) were more salt tolerance than the local cultivar (Tamooze) at germination stage under salinity conditions. The results of the amplification of the TaSC gene showed that there is a single clear band with 100 bp was appeared only in selected genotypes, while it was absent in local cultivar and negative control. Also the results of real time polymerase chain reaction amplification showed that there are differences in TaSC gene expression among the selected genotypes, whilst there is no gene expression in the local cultivar under all salinity levels. The threshold cycle (C_T) values indicated that the selected genotypes differed significantly in their gene amount, compared with local cultivar and the quantity of the TaSC gene recorded for Dijilla, Furat and N3 genotypes were (1.909 X 10¹⁵, 1.45 X 10¹⁵ and 1.279 X 10¹⁵) respectively which leaded to cause significant differences between them in their gene expressions. The results reflected that TaSC gene is present in selected genotypes of wheat Dijilla, Furat and N3 and absent in local cultivar (Tamooze). In conclusion, the TaSC gene can be used as a genetic marker for the wheat genotypes under study.

Keywords