The ultrastructure of chloroplast and quantified antioxidant compounds such as α-tocopherol, ascorbic acid and beta carotene, semi quantitatively by comparing the peak areas of HPLC chromatograms, were affected considerably in response to exposure of Azolla caroliniana to UV-B radiation for 24 and 48 h. The results show that the most striking effect of the enhanced UV-B treatment on the ultrastructure of A. caroliniana was observed after 48 h as markedly widened chloroplast area occupied by large amount of plastoglobuli in stressed fronds. Prolonged UV-B exposure of A. caroliniana for 48 h resulted in a reduction in peaks area for for α-tocopherol, ascorbic acid and beta-carotene. The results show that changes in the chromatogram of non-enzymatic antioxidants were correlated with the duration of exposure to UV-B radiation. These antioxidant metabolites play a vital role in protecting A. caroliniana to a certain limit against UV-B radiation.
Mostafa, E., & Ibrahim, M. (2012). HPLC analysis of non-enzymatic antioxidants in Azolla caroliniana (Pteridopsida) subjected to UV-B. Egyptian Academic Journal of Biological Sciences, H. Botany, 3(1), 19-30. doi: 10.21608/eajbsh.2012.17003
MLA
E. M. Mostafa; M. M. Ibrahim. "HPLC analysis of non-enzymatic antioxidants in Azolla caroliniana (Pteridopsida) subjected to UV-B", Egyptian Academic Journal of Biological Sciences, H. Botany, 3, 1, 2012, 19-30. doi: 10.21608/eajbsh.2012.17003
HARVARD
Mostafa, E., Ibrahim, M. (2012). 'HPLC analysis of non-enzymatic antioxidants in Azolla caroliniana (Pteridopsida) subjected to UV-B', Egyptian Academic Journal of Biological Sciences, H. Botany, 3(1), pp. 19-30. doi: 10.21608/eajbsh.2012.17003
VANCOUVER
Mostafa, E., Ibrahim, M. HPLC analysis of non-enzymatic antioxidants in Azolla caroliniana (Pteridopsida) subjected to UV-B. Egyptian Academic Journal of Biological Sciences, H. Botany, 2012; 3(1): 19-30. doi: 10.21608/eajbsh.2012.17003
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