Document Type : Original Article
Authors
1
Agricultural Botany Department, Faculty of Agriculture (Saba Basha), Alexandria University, Alexandria 21531, Egypt.
2
Plant Protection and Biomolecular Diagnosis Department, ALCRI, City of Scientific Research and Technological Applications, New Borg El Arab City, Alexandria 21934, Egypt.
Abstract
Background: The bacterial pathogens Pectobacterium carotovorum, P. atrosepticum, Ralstonia solanacearum, and Streptomyces scabies caused several diseases in potatoes. To control these pathogens, many chemical methods are used, which harm the environment and have an effect on human health. Recently, instead of these hazard methods, biocontrol agents like plant extracts were developed and had a good sense of control. Methods: The plant extracts were prepared in methanol and acetone solvents, and the disk diffusion method was used in the bioassay. Results: In this study, we tested two natural extracts as eco-friendly biobactericides against potato pathogens P. carotovorum, P. atrosepticum, R. solanacearum, and S. scabies. The Juniperus phoenicea 25% methanol extract showed considerable antibacterial activity in vitro, with optimal doses at 3000, 5000, 7000, and 10000 ppm. The extract effectively suppressed P. atrosepticum isolate, with inhibition zones ranging from 11.00 to 22.33 mm at doses of 3000-10000 ppm. However, it had little impact until reaching 3000 ppm against R. solanacearum (8.67–10 mm) and P. carotovorum (6.33–9.67 mm) isolates. The extract also greatly inhibited S. scabies isolate growth, with a mean inhibition zone of 3.5 mm. At doses of 7000 ppm and 3000 ppm, Cicer arietinum 75% acetone leaf extract showed the maximum effectiveness against P. atrosepticum and P. carotovorum, with inhibition zone values of 10 mm and 8.33 mm, respectively. However, it had less effectiveness against S. scabies (7.33–10.00 mm) and R. solanacearum (8.00–9.00 mm) isolates. Overall, these natural extracts may fight potato pathogenic microorganisms.
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