Detection of Zucchini Yellow Mosaic Virus-Infected Squash Plants in Egypt

Nabil, Rokaia; Abdelkhalek, Ahmed; Behiry, Said; Younes, Hosny

doi:10.21608/eajbsh.2023.331127

Detection of Zucchini Yellow Mosaic Virus-Infected Squash Plants in Egypt

Document Type : Original Article

Authors

¹ Agricultural Botany Department, Faculty of Agriculture (Saba Basha), Alexandria University, Alexandria 21531, Egypt.

² Plant Protection and Biomolecular Diagnosis Department, ALCRI, City of Scientific Research and Technological Applications, New Borg El Arab City, Alexandria 21934, Egypt.

10.21608/eajbsh.2023.331127

Abstract

Background: The Zucchini yellow mosaic virus (ZYMV) is well recognized as a very significant viral pathogen that causes substantial yield reductions in cucurbitaceous crops on a global scale. Methods: To detect and identify ZYMV in squash plants we used the serology reaction, transmission electron microscope, and molecular methods. Results: ZYMV was first isolated from naturally infected squash plants in the Alexandria governorate of Egypt, where it caused mosaic, yellowing, and deformation of the leaves. In addition to a molecular diagnosis utilizing RT-PCR, the identification relied on serological responses with antisera to CMV, SqMV, and ZYMV. Using centrifugation and PEG, the virus was cleaned. The A260/280 and A280/260 ratios of the purified virus were 1.38 and 0.72, respectively, which are consistent with those of a nucleoprotein. 2.36 mg of pure virus was recovered from per 100g of infected leaf material. The virus particles, as seen under an electron microscope after a partial purification preparation technique, have a flexuous filamentous structure and measure around 750 nm in length and 12 nm in diameter. Finally, the molecular investigation established the identification of the ZYMV isolate after a specific antiserum with a titer of 1:2.56×10⁴was generated and tested at the third week.

Keywords