Two field experiments were carried out at The Faculty of Agriculture (Saba Basha), Alexandria University, Egypt during 2023/2024 and 2024/2025 seasons to assess the effectiveness of Bacillus subtilis as a biological control agent in reducing the incidence and severity of viral infections (BYMV) in faba bean and its effects on vegetative growth. The experiment was conducted in a greenhouse over two consecutive seasons using a randomized complete block design (RCBD) in three replicates, with foliar application of B. subtilis at concentrations of 0.5, 1.0, 1.5, and 2.0 g/L, along with a control significantly improved the vegetative growth and yield of faba bean, especially when applied before viral infection. The 1.5 g/L pre-infection treatment resulted in the highest level of effectiveness among all treatments. The results indicated that foliar application of B. subtilis extract increased values in plant height, number of pods, and pod weight during both seasons, suggesting strong preventive effects. In contrast, virus-infected plants without treatment had the lowest performance across all traits. Treatments applied for post- infection gave moderate improvements but were less effective than preventive applications. These findings highlight the role of B. subtilis as a biocontrol agent that enhances plant immunity and growth under stress. It is recommended to apply B. subtilis preventively at 1.5 g/L for optimal protection and productivity in faba bean cultivation. ELISA is a sensitive and reliable technique used to detect viral infections in plants by measuring viral protein concentrations. It helps evaluate the effectiveness of biological treatments in reducing virus presence
Noah, I., Zeid, A., Younis, H., & Gaber, M. (2025). Evaluation of Bacillus subtilis As a Biocontrol Agent Against Viral Pathogens in Faba Bean (Vicia faba). Egyptian Academic Journal of Biological Sciences, H. Botany, 16(2), 33-44. doi: 10.21608/eajbsh.2025.452047
MLA
Ibrahim N. A. M. Noah; Ahmed H. Zeid; Hosny A. Younis; Mona M. Y. Gaber. "Evaluation of Bacillus subtilis As a Biocontrol Agent Against Viral Pathogens in Faba Bean (Vicia faba)", Egyptian Academic Journal of Biological Sciences, H. Botany, 16, 2, 2025, 33-44. doi: 10.21608/eajbsh.2025.452047
HARVARD
Noah, I., Zeid, A., Younis, H., Gaber, M. (2025). 'Evaluation of Bacillus subtilis As a Biocontrol Agent Against Viral Pathogens in Faba Bean (Vicia faba)', Egyptian Academic Journal of Biological Sciences, H. Botany, 16(2), pp. 33-44. doi: 10.21608/eajbsh.2025.452047
VANCOUVER
Noah, I., Zeid, A., Younis, H., Gaber, M. Evaluation of Bacillus subtilis As a Biocontrol Agent Against Viral Pathogens in Faba Bean (Vicia faba). Egyptian Academic Journal of Biological Sciences, H. Botany, 2025; 16(2): 33-44. doi: 10.21608/eajbsh.2025.452047
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