Determination of Genetic Variation and Fingerprinting of Some Genotypes of (Prunus Armeniaca) In Salah Al-Din -Iraq

Aldoury, Hanan S. S. Y.; Alsugmiany, Rafea Z. M.

doi:10.21608/eajbsh.2022.273737

Determination of Genetic Variation and Fingerprinting of Some Genotypes of (Prunus Armeniaca) In Salah Al-Din -Iraq

Document Type : Original Article

Authors

¹ Salah Al-Din Directorate of Education - Ministry of Education – Iraq

² Biology Department, College of Science, Tikrit University.

10.21608/eajbsh.2022.273737

Abstract

This research included studying the genetic markers for nineteen cultivars of apricot, comprising: Khudrawi1, Heshari, Sharika, Saboni, Labibi, Zaghini, Zingili, Herfi, Aisha, Tariq1, Tariq2, Al-Mostafa, Pigeon eggs, Al-Qaissi, Laozi, Khudrawi2, Khawki, Khairi and Orange Hashari. Hereditarily, it was employed one of the markers that depend on PCR, was Randomly Amplified Polymorphic DNA (RAPD), in order to detect the genetic variation among cultivars and to find genetic distance and cumulative analysis for nineteen samples. DNA was isolated from the plant leaves, then the RAPD reactions were employed by using sixteen primers; the products then were electrophoresed on a 1.5% agarose gel. It was noticeable that the primer which gave more bands is OP D-18, it gave 154 bands; its efficiency was 10.9, while its differentiation capacity was11.1. The variety rate of the cultivars by using this primer was 100%. On the other hand, the less producible primer was OP H-16, it produced only 29 bands and had less efficiency (2), while its differentiation capacity was 2.1. The variety rate of the cultivars by using OP H-16 was100%. It must be mentioned that the summation of the unique bands that appeared by using primers was 28 bands distributed among the studied cultivars. Khudrawi2 was butter luck, it owned 12 unique bands. It’s clear that the genetic markers had a role in the morphological variation, isolation of apricots’ cultivars and classifying them. The genetic tree which depended on RAPD primers showed that the most relative cultivars were Tariq1 and Al-Mustafa from Al-Alam; their relative rate was (0.9209), so they are regarded as the most relative variety. Whereas, the maximum genetic distance was 0.3220, which appeared between Zengili and Khudrawi2. The next was 0.3446 between Sharika and Khudhrawi2, then 0.3672 between Khudhrawi2 and Tariq2.

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